期刊
CANCER LETTERS
卷 328, 期 1, 页码 120-125出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2012.08.033
关键词
G-CIMP; Primary glioblastoma; DNA methylation; IDH1 mutation; Prognosis
类别
资金
- National Natural Science Foundation of China [81201993, 81101901]
- National High Technology Research and Development Program of China (863) [2012AA02A508]
- International Cooperation Program [2012DFA30470]
- Jiangsu Province's Key Provincial Talents Program [RC2011051]
- Jiangsu Province's Key Discipline of Medicine [XK201117]
To date, the aberrations in the DNA methylation patterns that are associated with different prognoses of G-CIMP- primary GBMs remain to be elucidated. Here, DNA methylation profiling of primary GBM tissues from 13 long-term survivors (LTS: overall survival >= 18 months) and 20 short-term survivors (STS; overall survival <= 9 months) was performed. Then G-CIMP+ samples were excluded. The differentially expressed CpG loci were identified between residual 18 STS and 9 LTS G-CIMP- samples. Methylation levels of 11 CpG loci (10 genes) were statistically significantly lower, and 43 CpG loci (40 genes) were statistically significantly higher in the tumor tissues of LTS than those of STS G-CIMP- samples (P < 0.01). Of the 43 CpG loci that were hypermethylated in LTS G-CIMP- samples, 3 CpG loci localized in the promoter of ALDH1A3. Furthermore, using an independent validation cohort containing 37 primary GBM samples without IDH1 mutation and MGMT promoter methylation, the hypermethylation status of ALDH1A3 promoter predicted a better prognosis with an accompanied low expression of ALDH1A3 protein. Taken together, our results defined prognosis-related methylation signatures systematically for the first time in G-CIMP- primary GBMs. ALDH1A3 promoter methylation conferred a favorable prognosis in G-CIMP- primary GBMs. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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