期刊
CANCER LETTERS
卷 334, 期 2, 页码 338-345出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2012.07.002
关键词
CD44; Hyaluronic acid; Nanostructured lipid carriers; Paclitaxel; Tumor target
类别
资金
- Special Funds for Independent Innovative Natural Sciences Research of Shandong University [2010JC019]
The aim of our study was to develop hyaluronic acid-coated, paclitaxel-loaded, nanostructured lipid carriers (HA-NLCs) prepared via electrostatic attraction for delivering paclitaxel (PTX) to tumors over-expressing CD44. First, cationic PTX-NLC was prepared by melt emulsion technology. Then, PTX-NLC were coated with hyaluronic acid (HA). The in vitro release of PTX was evaluated by the dialysis method. This analysis showed that PTX was released more slowly from HA-NLC than from Taxol(R). The in vitro cytotoxicity of HA-NLC was investigated using the MTT method in B16, CT26 and HCT116 cell lines. The results showed that the cytotoxicity of HA-NLC against these three cancer cell lines was superior to that of Taxol(R). The in vivo antitumor effect, the pharmacokinetics and the tissue distribution of HA-NLC were all evaluated in B16-bearing Kunming mice. The results showed that HA-NLC was better tolerated and had increased antitumor activity in B16-bearing Kunming mice compared with Taxol(R). Furthermore, HA-NLC could prolong the circulation time of PTX in blood and increase the accumulation of PTX in the tumor. Therefore, HA-NLC prepared via electrostatic attraction was an effective carrier for delivering PTX to tumors overexpressing CD44. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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