期刊
SPINAL CORD
卷 45, 期 1, 页码 15-24出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.sc.3101943
关键词
neural stem cells; Schwann cells; neurotrophin-3; transplantation; spinal cord injury; regeneration
Study design: An animal model of transected spinal cord injury (SCI) was used to test the hypothesis that cografted neural stem cells (NSCs) and NT-3-SCs promote morphologic and functional recoveries of injured spinal cord. Objective: To explore whether cotransplant of NSCs and NT-3-SCs could promote the injured spinal cord repair. Setting: Zhongshan Medical College, Sun Yat-sen University, PR China. Methods: Female Sprague - Dawley (SD) rats weighing on 200 - 220 g were used to prepare SCI models. The spinal cord was transected between T-9 and T-10, then NSCs, SCs + NSCs, LacZSCs + NSCs, or NT-3-SCs + NSCs were grafted into the transected site. Results: (1) Part of NSCs could differentiate to neuron-like cells in the transected site and the percentage of differentiation was NT-3-SCs + NSCs group > SCs + NSCs group > NSCs group. (2) In the grafted groups, there were 5-HT, CGRP, and SP positive nerve fibres within the transected site. Some. uorogold (FG)-labeled cells were found in the spinal cord rostral to the transected site, the red nuclei and the inner pyramidal layer of sensorimotor cortex. (3) The cells grafted could enhance the injured neurons survival in inner pyramidal layer of sensorimotor cortex, red nuclei of midbrain, and Clark's nuclei of spinal cord's L1 segment, could decrease the latency and increase the amplitude of cortical somatosensory evoked potential (CSEP) and cortical motor evoked potential (CMEP), and could promote partly structural and functional recovery of the SCI rats. Conclusion: These results demonstrate that cografted NT-3-SCs and NSCs is a potential therapy for SCI.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据