期刊
CANCER LETTERS
卷 328, 期 2, 页码 252-260出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2012.09.021
关键词
Hop; Cell migration; Pseudopodia; RhoC; RNA interference
类别
资金
- Medical Research Council (MRC)
- Cancer Research Initiative of South Africa (CARISA)
- South African National Research Foundation (NRF)
- NRF
The Hsp90/Hsp70 organising protein (Hop) is a co-chaperone that mediates the interaction of Hsp90 and Hsp70 molecular chaperones during assembly of Hsp90 complexes in cells. Formation of Hsp90 complexes is a key intermediate step in the maturation and homeostasis of oncoproteins and several hormone receptors. In this paper, we demonstrate that knockdown of Hop decreased migration of Hs578T and MDA-MB-231 breast cancer cells. Hop was identified in isolated pseudopodia fractions; it colocalised with actin in lamellipodia, and co-sedimented with purified actin in vitro. Knockdown of Hop caused a decrease in the level of RhoC GTPase, and significantly inhibited pseudopodia formation in Hs578T cells. Our data suggest that Hop regulates directional cell migration by multiple unknown mechanisms. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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