期刊
CANCER LETTERS
卷 316, 期 1, 页码 31-38出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2011.10.015
关键词
Lung cancer; Gene therapy; CEA; HSV-TK/CD; Killing effect
类别
资金
- National Natural Sciences Foundation of China [30471997]
- National Institute of Health [K12HD055881]
- Cure(R) Career Catalyst Research Grant [KG090510]
The application of gene therapy in cancer treatment is limited by non-specific targeting. In the present study, we constructed a recombinant plasmid, containing a carcinoembryonic antigen (CEA) promoter and double suicide genes thymidine kinase (TK) and cytosine deaminase (CD), henceforth referred to as pCEA-TK/CD. Our results showed that the CEA promoter can specifically drive target gene expression in CEA-positive lung cancer cells. In the presence of prodrugs 5-flucytosine and ganciclovir, pCEA-TK/CD transfection decreased inhibitory concentration 50 and increased apoptosis and cyclomorphosis. Our result suggests that gene therapy using pCEA-TK/CD may be a promising new approach for treating lung cancer. Crown Copyright (C) 2011 Published by Elsevier Ireland Ltd. All rights reserved.
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