Dendritic cells engineered to express GITRL enhance therapeutic immunity in murine Lewis lung carcinoma

Glucocorticoid-induced tumor necrosis factor receptor and its ligand (GITRL) are critic. involved in the regulation of immune response. In this study, we aimed to generate marrow-derived dendritic cells (BMDCs) transfected with recombinant adenovi expressing GITRL (pAd-GITRL-BMDCs) and explore their therapeutic efficacy in mut Lewis lung carcinoma. In vitro, pAd-GITRL-BMDCs greatly enhanced effector T cells prc eration but markedly abrogate the suppression of Treg cells. Moreover, vaccination it pAd-GITRL-BMDCs significantly retarded tumor growth, which was accompanied It. increased IFN-gamma-producing CD8+ T cells and markedly decreased Treg cells in vivo. Th findings suggest GITRL could enhance the immune stimulation of DC and might facilit the potential development of DCs-based anti-tumor therapies. (c) 2010 Elsevier Ireland Ltd. All rights reserved