期刊
CANCER LETTERS
卷 305, 期 1, 页码 50-57出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2011.02.025
关键词
Glioblastoma; VEGF; PDGF; Glioblastoma cell line; Drug development; Model system
类别
资金
- Medical Research Council
- Royal College of Surgeons of Edinburgh
- NIHR Cambridge Biomedical Research Centre
- Medical Research Council [G108/507] Funding Source: researchfish
- MRC [G108/507] Funding Source: UKRI
We provide evidence that six glioblastoma cell lines derived and maintained under serum-free conditions secrete VEGF and four also expressed VEGF(R2). Expression of VEGFR2 was associated with reduced proliferation in response to anti-VEGF antibodies. Spontaneous loss of VEGF(R2) over passage was associated with loss of this anti-proliferative effect. Gain of expression of VEGF(R2) was not associated with the acquisition of responsiveness to anti-VEGF antibodies. Secretion of PDGF was absent in 5/6 of our cell lines and none of the cell lines had reduced proliferation in response to anti-PDGF antibodies suggesting that PDGF autocrine signalling was unlikely to be significant in tumour proliferation. These data are consistent with published clinical trials suggesting that glioblastoma cell lines derived under serum-free conditions have the potential for use in drug screening and individualising patient therapy. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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