期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 31, 期 5, 页码 499-510出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2006.08.007
关键词
rainbow trout; innate immunity; gene expression; gene duplication; toll-like receptor; Atlantic salmon; pathogen
Structure and function of factors contributing to the innate immune system of lower vertebrates, including fish are only sparsely characterized. We retrieved with RT-PCR cDNA copies of two closely related Toll-like receptors (TLR) from liver RNA of the rainbow trout (Oncorhynchus mykiss). The cDNA sequences are homologous to 95.6%. The phylogenetic analysis of their deduced amino acid sequences places these twin factors closely to other known TLRs from fish. The twin factors are equally expressed in all tissues analysed, most abundantly in spleen and head kidney and lowest in adipose tissue. Formalin-inactivated Aeromonas salmonicida pathogens induce their expression up to eight-fold in vitro in peripheral blood lymphocytes and in tissues from spleen and head kidney. Our sequence information will be useful to establish expression constructs for these factors necessary to analyse the pathogen specific signal transduction activating the innate immune defence in fish. (c) 2006 Elsevier Ltd. All rights reserved.
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