期刊
CANCER LETTERS
卷 294, 期 2, 页码 204-210出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2010.02.001
关键词
Autophagy; Apoptosis; Beclin1; Anti-cancer
类别
资金
- Natural Science Foundation of Fujian Province of China [2009J01113]
The purpose of this study was to investigate whether the autophagy-related gene. Beclin1, plays a role in the regulation of chemosensitivity to anti-cancer drugs in cervical cancer CaSki cells. Expression of the Beclin1 protein was up-regulated in pcDNA3.1-Bec transfectants and led to cell arrest in the G(0)/G(1) phase of the cell cycle. The MTT assay indicated that over-expression of Beclin1 sensitized CaSki cells to chemotherapeutic drugs (cisplatin, paclitaxel, 5-fluorouracil, and epirubicin) and induced greater degrees of cytotoxicity than vector-only controls. After treatment with anti-cancer drugs, flow cytometric analysis indicated that the Beclin1-transfected group showed a greater increase in apoptosis than did the non-transfected group. Furthermore, pSUPER-Bec transfectants did not lead to a significant increase of resistance to each of these anti-cancer drugs. These results suggest that Beclin1 plays an important role in the regulation of potent anti-tumor activity, and over-expression of Beclin1 in CaSki cells may enhance apoptosis signaling induced by anti-cancer drugs. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
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