期刊
CANCER LETTERS
卷 281, 期 1, 页码 32-41出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2009.02.022
关键词
Osteosarcoma; Mesenchymal stem cells; SDF-1; CCL5; Metastasis
类别
资金
- Shanghai Science and Technology Development Fund [064307055]
- Program for New Century Excellent Talents in University [NCET-06-0401]
- Program for Shanghai Key Laboratory of Orthopaedic Implant [08DZ2230330]
In an effort to study the interaction between MSCs and osteosarcoma, we established an animal model of primary osteosarcoma in nude mice using Saos-2 cells. hMSCs, labeled with adv-GFP, were injected through the caudal vein. We observed that exogenous hMSCs targeted the osteosarcoma site and promoted its growth and pulmonary metastasis in vivo. To elucidate the underlying mechanisms, we employed transwell, neutralization antibody and MTT assays in vitro. hMSCs migrated toward the conditioned medium from Saos-2 cells, and SDF-1 was involved in this migration. Likewise, Saos-2 cells migrated toward the conditioned medium from hMSCs and CCL5 played an important role in this migration. Furthermore, proliferation of Saos-2 cells was enhanced by the conditioned medium from hMSCs and CCL5 was at least partly responsible for this enhancement. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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