期刊
BIOELECTROCHEMISTRY
卷 70, 期 1, 页码 180-184出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2006.03.014
关键词
anthrax; tuberculosis; electrochemical; bacteriophage; detection
The rapid and reliable detection of pathogenic microorganisms is an important issue for the safety and security of our society. Here we describe the use of a sensitive, inexpensive, amperometric, phage-based biosensor for the detection of extremely low concentrations of Bacillus cereus and Allycobacterium smegmatis as models for Bacillus anthracis (the causative agent of anthrax) and for Mycobacterium tuberculosis (the causative agent of tuberculosis), respectively. The detection procedure developed here enabled the determination of bacteria at a low concentration of 10 viable cells/mL within 8 h. This experimental setup allows the simultaneous analysis of up to eight independent samples, using disposable screen-printed electrodes. (c) 2006 Elsevier B.V. All rights reserved.
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