期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 31, 期 11, 页码 1159-1167出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2007.03.002
关键词
immune response; encapsulation; neuroglian; integrin; immunoglobulin (1g) domains
资金
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL064657] Funding Source: NIH RePORTER
Neuroglian, a member of the L1 family of cell adhesion molecules (L1-CAMs), is expressed on surfaces of granular cells and a subset of large plasmatocytes of Manduca sexta that act as foci for hemocyte aggregation during the innate immune response. Neuroglian expressed on surfaces of transfected Sf9 cells induced their homophilic aggregation, with the aggregation being abolished in the presence of recombinant immunoglobulin (Ig) domains of neuroglian. Neuroglian and its Ig domains also can interact with hemocyte-specific integrin (HS integrin) as demonstrated with an enzyme-linked immunoassay and a surface plasmon resonance (SPR) assay. Neuroglian double-stranded (ds) RNA not only depresses expression of neuroglian in hemocytes but also depresses the cell-mediated encapsulation response of these hemocytes to foreign surfaces. After injection of a monoclonal antibody (MAb 3B11) into M. sexta larvae that recognizes the Ig domains of neuroglian, the cell-mediated encapsulation response of hemocytes was likewise inhibited. The Ig domains of neuroglian are involved in both homophilic and heterophilic interactions, and subsets of these six different Ig domains may affect different functions of neuroglian. (C) 2007 Elsevier Ltd. All rights reserved.
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