期刊
CANCER LETTERS
卷 278, 期 2, 页码 164-173出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2009.01.013
关键词
Prominin; Cancer stem cells; DNA sequencing; Glycolipid-binding domain; Ganglioside; GM1; CD133; Stem cells; Ganglioside; Colon cancer cells; Glycolipid
类别
Prominin 1/CD133 is a marker of transplantable cancer stem cells. We have generated antipeptide antibodies against a N-terminal epitope of CD133 belonging to a ganglioside-binding domain. The labelling of colon cancer cells with these antibodies was inhibited by various gangliosides including GM1 and GD3, but not GT1b. CD133 immunolabelling progressively decreased to undetectable levels in post-confluent cultures, possibly through ganglioside-mediated epitope masking since the staining was partially recovered after chemical disruption of lipid rafts. We suggest that selected gangliosides could modulate the accessibility of CD133 and regulate cell-cell contacts involving CD133* stem cells at the earliest steps of tumour development. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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