期刊
CANCER LETTERS
卷 263, 期 2, 页码 280-290出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2008.01.013
关键词
heat shock protein 70; autoantibody; matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry; proteomics; esophageal cancer
类别
Detection of novel tumor-related antigens and autoantibodies in cancer patients is expected to facilitate the diagnosis of early-stage malignant tumor and establish effective new immunotherapies. The purpose of this study was to identify novel tumor antigens in an esophageal squamous cell carcinoma (ESCC) cell line (TE-2) and related autoantibodies in sera from patients with ESCC using a proteomics-based approach. TE-2 proteins were separated by two-dimensional polyacrylamide gel electrophoresis, followed by Western blot analysis in which sera from patients with ESCC, healthy controls and patients with other cancers were tested for primary antibodies. Positive spots were excised from silver-stained gels and analyzed by matrix-assisted laser disorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). Sera from patients with ESCC yielded multiple spots, one of which was identified as heat shock protein 70 (Hsp70) by MALDI-TOF/TOF-MS. Concentrations of serum Hsp70 autoantibody were significantly higher for patients with ESCC (mean, 0.412 +/- 0.096mg/ml) than for patients with gastric. (0.236 +/- 0.112mg/ml, P < 0.001) or colon cancer (0.231 +/- 0.120 mg/ml, P < 0.001) or healthy individuals (0.207 +/- 0.055 mg/ml, P < 0.001) by enzyme-linked immunosorbent assay. We have identified an autoantibody against Hsp70 in ESCC patients. The proteomic approach implemented herein offers a powerful tool for identifying novel serum markers that may display clinical utility against cancer. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据