A novel treatment of contact dermatitis by topical application of phospholipase A(2) inhibitor: A double-blind placebo-controlled pilot study

Phospholipase A(2) hydrolyzes membrane phospholipids releasing arachidonic acid and lysophospholipids. These are key precursors of inflammatory mediators, such as prostaglandins, leukotrienes, thromboxanes and PAF, in numerous inflammatory/allergic diseases, including skin inflammation. Accordingly, inhibition of PLA(2) has long been postulated as a potentially potent antiinflammatory therapy. In the present study we tested the effect of a novel PLA(2) inhibitor on contact dermatitis in human subjects. A double-blind, placebo-controlled pilot study was conducted on contact dermatitis patients (n=11) treated with the inhibitor-containing topical preparation (1% cream). Disease severity was assessed by physician's assessment before treatment (day 0) as well as after 14-days and 30-days. Patients treated with 1% PLA2 inhibitor-containing cream showed a 69.9% reduction in disease score while placebo-treated patients showed a reduction of 36.5% with p=0.0024. The clear improvement in the disease score of inhibitor-treated patients supports the involvement of PLA(2) activity in skin inflammation and the therapeutic prospective of its inhibition.