期刊
CANCER IMMUNOLOGY IMMUNOTHERAPY
卷 64, 期 2, 页码 225-235出版社
SPRINGER
DOI: 10.1007/s00262-014-1629-5
关键词
Adoptive cell therapy; Natural killer cells; Migration; CXCL10; CXCR3
资金
- Swedish Research Council [522-208-2377]
- Swedish Cancer Society [CAN 2012/474]
- FP7 Marie Curie re-integration Grant [246759]
- Cancer Society in Stockholm [121132]
- Swedish Society of Medicine [325751]
- Karolinska Institutet
- Jeanssons Stiftelser
- Ake Wibergs Stiftelse
- Magnus Bergvalls Stiftelse
- Fredrik och Ingrid Thurings Stiftelse
- Stiftelsen Clas Groschinskys Minnesfond
- Division of Intramural Research at the Hematology Branch of the National Heart Blood and Lung Institute
Adoptive infusion of natural killer (NK) cells is being increasingly explored as a therapy in patients with cancer, although clinical responses are thus far limited to patients with hematological malignancies. Inadequate homing of infused NK cells to the tumor site represents a key factor that may explain the poor anti-tumor effect of NK cell therapy against solid tumors. One of the major players in the regulation of lymphocyte chemotaxis is the chemokine receptor chemokine (C-X-C motif) receptor 3 (CXCR3) which is expressed on activated NK cells and induces NK cell migration toward gradients of the chemokine (C-X-C motif) ligand (CXCL9, 10 and 11). Here, we show that ex vivo expansion of human NK cells results in a tenfold increased expression of the CXCR3 receptor compared with resting NK cells (p = 0.04). Consequently, these NK cells displayed an improved migratory capacity toward solid tumors, which was dependent on tumor-derived CXCL10. In xenograft models, adoptively transferred NK cells showed increased migration toward CXCL10-transfected melanoma tumors compared with CXCL10-negative wild-type tumors, resulting in significantly reduced tumor burden and increased survival (median survival 41 vs. 32 days, p = 0.03). Furthermore, administration of interferon-gamma locally in the tumor stimulated the production of CXCL10 in subcutaneous melanoma tumors resulting in increased infiltration of adoptively transferred CXCR3-positive expanded NK cells. Our findings demonstrate the importance of CXCL10-induced chemoattraction in the anti-tumor response of adoptively transferred expanded NK cells against solid melanoma tumors.
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