期刊
GENESIS
卷 45, 期 1, 页码 51-58出版社
WILEY
DOI: 10.1002/dvg.20264
关键词
bacterial recombineering; SALL4; Okihiro syndrome; mouse; organogenesis; embryonic stem cells
资金
- Medical Research Council [MC_qA137918] Funding Source: researchfish
- Medical Research Council [MC_QA137918, G0300212] Funding Source: Medline
- Wellcome Trust [077187] Funding Source: Medline
- MRC [MC_qA137918, G0300212] Funding Source: UKRI
SALL4 is a homologue of the Drosophila homeotic gene spalt, a zinc finger transcription factor, required for inner cell mass proliferation in early embryonic development. It also interacts with other transcription factors to control the development of the anorectal region, kidney, heart, limbs, and brain. Truncating mutations in SALL4 cause Okihiro syndrome, manifest as Duane anomaly, radial ray defects and sensorineural and conductive deafness. We report the characterization of a novel murine Sall4 null allele created by bacterial recombineering in ES cells. Homozygous mutant mice exhibit early embryonic lethality. Heterozygous mutant mice recapitulate phenotypic features of Okihiro syndrome including deafness, lower anogenital tract abnormalities, renal hypoplasia, anencephaly, Hirschprung's disease, and skeletal defects. This phenotype shows important differences in cardiac and ear manifestations to previously characterized Sall4 mutant alleles and should prove useful for the investigation of the influence of modifier alleles and protein interactions on the transcriptional regulatory function of Sall4.
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