期刊
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
卷 23, 期 6, 页码 1007-1017出版社
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/1055-9965.EPI-13-1256
关键词
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资金
- Intramural Research Program of the NIH
- NIEHS [Z01-ES-49019, Z01-ES-046008]
- Norwegian Ministry of Health
- Ministry of Education and Research
- NIH/NIEHS [NO-ES-75558]
- NIH/NINDS [1 UO1 NS 047537-01]
- Norwegian Research Council/FUGE [151918/S10]
- Faculty of Medicine and Dentistry, University of Bergen, Norway
Background: Maternal smoking in pregnancy is associated with adverse health outcomes in children, including cancers; underlying mechanisms may include epigenetic modifications. Using Illumina's 450K array, we previously identified differential DNA methylation related to maternal smoking during pregnancy at 26 CpG sites (CpGs) in 10 genes in newborn cord bloods from the Norwegian Mother and Child Cohort Study (MoBa). Whether these methylation signals in newborns reflect in utero exposure only or possibly epigenetic inheritance of smoking-related modifications is unclear. Methods: We therefore evaluated the impact of the timing of mother's smoking (before or during pregnancy using cotinine measured at 18 weeks gestation), the father's smoking before conception, and the grandmother's smoking during her pregnancy with the mother on methylation at these 26 CpGs in 1,042 MoBa newborns. We used robust linear regression, adjusting for covariates, applying Bonferroni correction. Results: The strongest and only statistically significant associations were observed for sustained smoking by the mother during pregnancy through at least gestational week 18 (P < 1.6 x 10(-5) for all 26 CpGs). We observed no statistically significant differential methylation due to smoking by the mother before pregnancy or that ceased by week 18, father's smoking before conception, or grandmother's smoking while pregnant with the mother. Conclusions: Differential methylation at these CpGs in newborns seems to reflect sustained in utero exposure rather than epigenetic inheritance. Impact: Smoking cessation in early pregnancy may negate effects on methylation. Analyses of maternal smoking during pregnancy and offspring health outcomes, including cancer, limited to ever smoking might miss true associations. (C) 2014 AACR.
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