期刊
ANALYTICAL METHODS
卷 7, 期 7, 页码 3164-3168出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ay00384a
关键词
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资金
- Natural Science Research Project of Education Department of Anhui Province [KJ2012Z399]
- Natural Science Foundation of Anhui Province [1208085QB33, 1408085MB40, 1408085QB41]
- National Nature Science Foundation of China [21271136]
- Natural Science Research Key Project of Education Department of Anhui Province [KJ2012A267]
- Opening Project of Anhui Key Laboratory of Spin Electron and Nanomaterials (Cultivating Base) [2014YKF52]
- Suzhou University Cultivate Outstanding Talent [2014XQNRL005]
A label-free electrochemical sensor for detecting the CaMV35S transgene gene sequence was reported based on a ZrO2/poly(thionine)/multiwalled carbon nanotubes modified glassy carbon electrode (ZrO2/PTH/MWCNTs/GCE). Probe oligonucleotides (p-ssDNA) were immobilized on the ZrO2 thin films via the strong affinity between the phosphate groups and ZrO2. Differential pulse voltammetry (DPV) was used to monitor DNA hybridization. Upon hybridization electrochemical signal changes were generated at the PTH-solution interface. Compared with noncomplementary target ssDNA, an obvious decrease in the peak current was observed upon p-ssDNA hybridization with complementary target ssDNA. The sensor exhibited an excellent selectivity for distinguishing three-mismatched with complementary target ssDNA and a high sensitivity for detecting complementary target ssDNA with the detection limit of 0.34 pM (S/N = 3). In addition, the sensor showed an excellent reproducibility and stability, and applied to determine the CaMV35S transgene gene sequence in samples with satisfactory results.
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