An anatomical study of the haustoria of Rhinanthus minor attached to roots of different hosts

Roots of a range of potential hosts responded differently when Rhinanthus minor attempted to form haustoria. Roots of Fabaceae show the weakest reaction as apart from slight lignification, no reaction was observed at the interface between the endophyte and the cortical tissue of the host root. Grass roots react with strong lignification of all cells within the stele with the exception of a small number of phloem cells whilst the endodermis fully enters the tertiary stage. In the case of Phleum bertolonii the cortical cells also become lignified. The lignification is even observed in the host root tissue in a distance of about I mm from the haustorium (both apically and basipetally). In the case of Leucanthemum vulgare, strong suberisation can be observed in the cell walls of the interface between endophyte (tip of the sucker) and host. Plantago lanceolata exhibits the strongest reactions against the haustorial tissues. Cells of the interface between the endophyte and the host cortex are completely destroyed, as well as a few cell layers outside the central xylem cylinder, even in some distance from the haustorium. Thus, host xylem is completely isolated from the haustorium in this case. Extraction of sap from xylem vessels is likely to be drastically impaired in such a situation. (C) 2007 Elsevier GmbH. All rights reserved.