期刊
JOURNAL OF SYNCHROTRON RADIATION
卷 14, 期 -, 页码 73-83出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S0909049506044177
关键词
radioprotectants; radiation damage; macromolecular cryocrystallography
Radiation damage continues to present a problem to crystallographers using cryocooled protein crystals at third-generation synchrotrons. Free-radical scavengers have been suggested as a possible means of reducing the rate of this damage. The screening of a large number of potential radioprotectants was undertaken with an online microspectrophotometer using cystine and cysteine to model protein disulfide bonds and thiol groups, respectively. Oxidized alpha-lipoic acid was tested as a possible model disulfide bond. The evidence for the effectiveness of ascorbate as a radioprotectant was strengthened, and quinone, 2,2,6,6-tetramethyl-4-piperidone, and reduced dithiothreitol showed promise as radioprotectants.
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