期刊
BIOCATALYSIS AND BIOTRANSFORMATION
卷 25, 期 1, 页码 109-112出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10242420601141721
关键词
acetovanillone; coniferyl alcohol; dihydroferulic acid; ferulic acid; Rhizopus oryzae; 4-vinyl guaiacol
Microbial transformation of ferulic acid to acetovanillone was studied using growing cells of Rhizopus oryzae. Ferulic acid was added to the growing medium (0.5 g L-1) and incubated for 12 days. The progress of formation of metabolites was monitored by GC and GC-MS after extraction with ethyl acetate. The major metabolite was acetovanillone with minor metabolites formed, such as dihydroferulic acid, coniferyl alcohol and dihydroconiferyl alcohol. Traces of metabolites (<= 1-3%), such as vanillin, vanillyl alcohol, vanillic acid and phenyl ethyl alcohol, were also produced. Formation of 4-vinyl guaiacol increased from day 1 (12.4%), reaching a maximum on day 4 (31.7%), and reducing to a minimum on day 12 (3.1%). The formation of acetovanillone increased only from day 2 onward, and reached a maximum (49.2%) on day 12. The optimum concentration of ferulic acid to be added into the medium was found to be only 0.5 g L-1, as any increase in concentration (0.75 and 1.0 g L-1) precipitated the precursor, resulting in no further degradation.
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