期刊
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
卷 21, 期 7, 页码 1105-1114出版社
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/1055-9965.EPI-12-0236
关键词
-
资金
- Pfizer
- NIH [DA U01 02830, DA 02277, CA 78603, DA 12393, R25 CA 113710, NCRR UCSF CTSI UL1 RR 024131]
- Canadian Institute of Health (CIHR) [86471, 115145, TMH-109787]
- Ontario Mental Health Foundation (OMHF)
- Centre for Addiction and Mental Health (CAMH)
- Canada Foundation for Innovation Leader Opportunity Fund (CFI-LOF) [19229]
- CAMH
- CRC
Background: The nicotine metabolite ratio (NMR or 3-hydroxycotinine/connine) has been used to phenotype CYP2A6-mediated nicotine metabolism. Our objectives were to analyze (i) the stability of NMR in plasma, saliva, and blood in various storage conditions, (ii) the relationship between NMRs derived from blood, plasma, saliva, and urine, and (iii) the reproducibility of plasma NMR in ad libitum cigarette smokers. Methods: We analyzed data from four clinical studies. In studies 1 and 2, we assessed NMR stability in saliva and plasma samples at room temperature (similar to 22 degrees C) over 14 days and in blood at 4 C for up to 72 hours. In studies 2 and 3, we used Bland-Altman analysis to assess agreement between blood, plasma, saliva, and urine NMRs. In study 4, plasma NMR was measured on six occasions over 44 weeks in 43 ad libitum smokers. Results: Reliability coefficients for stability tests of NMR in plasma and saliva at room temperature were 0.97 and 0.98, respectively, and 0.92 for blood at 4 degrees C. Blood NMR agreed consistently with saliva and plasma NMRs but showed more variability in relation to urine NMR. The reliability coefficient for repeated plasma NMR measurements in smokers was 0.85. Conclusion: The NMR is stable in blood, plasma, and saliva at the conditions tested. Blood, plasma, and saliva NMRs are similar whereas urine NMR is a good proxy for these NMR measures. Plasma NMR was reproducible over time in smokers. Impact: One measurement may reliably estimate a smoker's NMR for use as an estimate of the rate of nicotine metabolism. Cancer Epidemiol Biomarkers Prey; 21(7); 1105-14. (C)2012 AACR.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据