期刊
JOURNAL OF MICROSCOPY-OXFORD
卷 225, 期 3, 页码 209-213出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1365-2818.2007.01746.x
关键词
calcium imaging; fluorescence lifetime; two-photon microscopy
类别
We analyzed the suitability of various Ca2+-indicator dyes for quantitative two-photon fluorescence-lifetime imaging. Although fura-2, fluo-3, BTC and calcein did not show useful Ca2+-dependent lifetime changes, calcium orange, calcium green-1, oregon green-2 and -5N, as well as magnesium green allowed to quantify the Ca2+-free and Ca2+-bound dye fractions by a double-exponential lifetime analysis. For the latter dyes, we derived calibration formalisms that correct for lifetime distortions by dye impurities and Ca2+-dependent extinction coefficients.
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