期刊
ANALYTICAL METHODS
卷 7, 期 23, 页码 10026-10031出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ay02131f
关键词
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资金
- National Natural Science Foundation of China [21375018]
- Natural Science Foundation of Fujian Province [2014J01402]
A facile strategy based on the synergistic effect of molecular imprinting and boronate affinity was proposed for glycoprotein imprinting. Polydopamine (PDA)-coated boronic acid-functionalized molecularly imprinted silica nanoparticles (MIPs) were prepared by the thiol-ene click reaction using SiO2 as the core, 3-acrylamidophenyl boronic acid (AAPBA) as the functional monomer, and horseradish peroxidase (HRP) as the glycoprotein template. A well defined core-shell structure of MIPs was obtained after self-polymerization of dopamine (DA) on the surface of HRP-immobilized silica nanoparticles (NPs). The polymerization conditions and adsorption behavior were investigated in detail in order to obtain the highest selectivity and binding capacity. Under the optimized conditions, the HRP-MIPs showed higher binding affinity towards HRP than non-imprinted nanoparticles (NIPs), and the corresponding adsorption capacity (Q) and imprinted factor (alpha) reached 0.58 mmol g(-1) and 2.6, respectively. The specificity for HRP recognition was evaluated with a competitive experiment, and the results indicated that the HRP-MIPs had higher selectivity for the template. The good features of the HRP-MIPs facilitated selective isolation and enrichment of trace HRP from human serum. In addition, the stability and regeneration were also investigated, which indicated that the HRP-MIPs had excellent reusability.
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