期刊
CANCER CYTOPATHOLOGY
卷 122, 期 12, 页码 914-920出版社
WILEY-BLACKWELL
DOI: 10.1002/cncy.21473
关键词
cervical cancer screening; p16; Ki-67; human papillomavirus; reproducibility; cytology; Papanicolaou test
资金
- Roche
- BD
- GE Healthcare
- Cepheid
- Hologic
- Qiagen
- ClearPath
- Guided Therapeutics
BACKGROUNDDual-stain cytology for p16 and Ki-67 has been proposed as a biomarker in cervical cancer screening. The authors evaluated the reproducibility and accuracy of dual-stain cytology among 10 newly trained evaluators. METHODSIn total, 480 p16/Ki-67-stained slides from human papillomavirus-positive women were evaluated in masked fashion by 10 evaluators. None of the evaluators had previous experience with p16 or p16/Ki-67 cytology. All participants underwent p16/Ki-67 training and subsequent proficiency testing. Reproducibility of dual-stain cytology was measured using the percentage agreement, individual and aggregate values, as well as McNemar statistics. Clinical performance for the detection of cervical intraepithelial neoplasia grade 2 or greater (CIN2+) was evaluated for each individual evaluator and for all evaluators combined compared with the reference evaluation by a cytotechnologist who had extensive experience with dual-stain cytology. RESULTSThe percentage agreement of individual evaluators with the reference evaluation ranged from 83% to 91%, and the values ranged from 0.65 to 0.81. The combined value was 0.71 for all evaluators and 0.73 for cytotechnologists. The average sensitivity and specificity for the detection of CIN2+ among novice evaluators was 82% and 64%, respectively; whereas the reference evaluation had 84% sensitivity and 63% specificity, respectively. Agreement on dual-stain positivity increased with greater numbers of p16/Ki-67-positive cells on the slides. CONCLUSIONSGood to excellent reproducibility of p16/Ki-67 dual-stain cytology was observed with almost identical clinical performance of novice evaluators compared with reference evaluations. The current findings suggest that p16/Ki-67 dual-stain evaluation can be implemented in routine cytology practice with limited training. Cancer (Cancer Cytopathol) 2014;122:914-920. (c) 2014 American Cancer Society. The authors evaluate the reproducibility and accuracy of p16/Ki-67 dual-stain cytology among 10 newly trained evaluators. The results indicate that the evaluation of p16/Ki-67 dual staining on cytology slides can be implemented in routine cytology practice with limited training and good to excellent reproducibility.
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