期刊
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
卷 196, 期 3, 页码 171-180出版社
SPRINGER
DOI: 10.1007/s00430-007-0041-6
关键词
lactoferrin; macrophage; IL-12; vaccine adjuvant
资金
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R42AI051050, R41AI051050] Funding Source: NIH RePORTER
- NIAID NIH HHS [R41AI51050-01, R41 AI051050-01A1, R42-AI051050-02, R41 AI051050, R42 AI051050-02, R42 AI051050] Funding Source: Medline
Lactoferrin possesses a wide range of immunomodulatory activities, including promotion of the delayed type hypersensitivity response (DTH) towards BCG (Bacillus Calmette Guerin) antigens. Addition of Lactoferrin as an adjuvant to the BCG vaccine was previously demonstrated to augment protection against subsequent mycobacterial challenge, with concomitant development of a strong T cell helper type 1 (T(H)1) immunity. Because generation of T(H)1 immunity is in large part dependent on the balance of monocytic pro- and anti-inflammatory cytokines, the effect of Lactoferrin on leukocytes was investigated. Lactoferrin enhanced proinflammatory responses in a dose-dependant manner from splenocyte and adherent (F4/80(+)) splenocyte populations, bone marrow derived monocytes (BMM), and J774A.1 cultured cells. In all scenarios tested, Lactoferrin induced a strong increase in the ratio of IL-12:IL-10 production from LPS stimulated cells. Examination of Lactoferrin effects on BCG infected J774A.1 cells and on BMM revealed similar immunomodulatory effects, with particularly strong increase in IL-12 production. Furthermore, immunization of mice with BCG admixed with Lactoferrin led to increased generation of CD4+ cells expressing IFN-gamma upon restimulation with BCG antigens. These results provide molecular evidence to support the role of Lactoferrin as an adjuvant candidate to augment development of DTH response to vaccine antigens.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据