期刊
JOURNAL OF ECONOMIC ENTOMOLOGY
卷 109, 期 1, 页码 299-306出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/jee/tov276
关键词
Bactrocera dorsalis; malathion; inheritance mode; realized heritability; biochemical mechanism
类别
资金
- Special Fund for Agro-scientific Research in the Public Interest [201203038]
- Natural Science Foundation of Chongqing (CSTC) [2013jjB0176]
- earmarked fund for Modern Agro-industry (Citrus) Technology Research System
- Foundation Project of Southwest University [SWU114049]
To better characterize the resistance development and therefore establish effective pest management strategies, this study was undertaken to investigate the inheritance mode and biochemical mechanisms of malathion resistance in the oriental fruit fly, Bactrocera dorsalis (Hendel), which is one of the most notorious pests in the world. After 22 generations of selection with malathion, the malathion-resistant (MR) strain of B. dorsalis developed a 34-fold resistance compared with a laboratory susceptible strain [malathion-susceptible (MS)]. Bioassay results showed that there was no significant difference between the LD50 values of malathion against the progenies from both reciprocal crosses (F-1-SR and F-1-RS). The degree of dominance values (D) was calculated as 0.39 and 0.32 for F-1-RS and F-1-SR, respectively. The logarithm dosage-probit mortality lines of the F-2 generation and progeny from the backcross showed no clear plateaus of mortality across a range of doses. In addition, Chi-square analysis revealed significant differences between the mortality data and the theoretical expectations. The realized heritability (h(2)) value was 0.16 in the laboratory-selected resistant strain of B. dorsalis. Enzymatic activities identified significant changes of carboxylesterases, cytochrome P450 (general oxidases), and glutathione S-transferases in MR compared with the MS strain of B. dorsalis. Taken together, this study revealed for the first time that malathion resistance in B. dorsalis follows an autosomal, incompletely dominant, and polygenic mode of inheritance and is closely associated with significantly elevated activities of three major detoxification enzymes.
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