期刊
CANCER CELL INTERNATIONAL
卷 14, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1475-2867-14-1
关键词
Lung cancer; Apoptosis; NSCLC; Ceramides; p-Akt; p-NF kappa B; survivin; cyclin A2
类别
资金
- National Science Council, Taiwan [NSC101-2313-B-037-001, NSC101-2622-B-037-002-CC3, NSC101-2320-B-037-046-MY3]
- ChiMei-KMU Joint Research Project
- NSYSU-KMU Joint Research Project [NSYSUKMU 102-28]
anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C-2-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C-2-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G(1) arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NF kappa B, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C-2-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells.
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