Persistently Activated Stat3 Maintains Constitutive NF-κB Activity in Tumors

NF-kappa B (ReIA) is constitutively active in many cancers, where it upregulates antiapoptotic and other oncogenic genes. While proinflammatory stimulus-induced NF-kappa B activation involves IKK-dependent nuclear translocation, mechanisms for maintaining constitutive NF-kappa B activity in tumors have not been elucidated. We show here that maintenance of NF-kappa B activity in tumors requires Stat3, which is also frequently constitutively activated in cancer. Stat3 prolongs NF-kappa B nuclear retention through acetyltransferase p300-mediated RelA acetylation, thereby interfering with NF-kappa B nuclear export. Stat3-mediated maintenance of NF-kappa B activity occurs in both cancer cells and tumor-associated hematopoietic cells. Both murine and human cancers display highly acetylated RelA, which is associated with Stat3 activity. This Stat3/NF-kappa B interaction is thus central to both the transformed and nontransformed elements in tumors.