期刊
JOURNAL OF ECONOMIC ENTOMOLOGY
卷 108, 期 6, 页码 2779-2788出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/jee/tov229
关键词
Chrysopa septempunctata; vitellogenin; RNA interference; function
类别
资金
- National Basic Research Program of China [2013CB127602]
- Agriculture Cooperative Research Fund [2011-G4]
Vitellogenin (Vg) is a precursor of major egg storage protein, vitellin (Vt), and plays primary roles in reproduction of oviparous vertebrates and invertebrates. Chrysopa septempunctata Wesmael is an important and common predator of various insect pests. Here, we first cloned C. septempunctata Vg gene CsVg. The complete CsVg cDNA was 5664 bp, which encodes an 1810-residues protein with a predicted molecula CsVg mRNA first appeared on day 4 after emergence, maximally accumulated on day 10, and then declined gradually. RNAi mediated by injection of dsRNA depleted CsVg transcripts, significantly reduced egg-laying amount, and decreased egg hatching rate, suggesting that CsVg functions through effects on egg production and hatching in C. septempunctata.
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