期刊
CANCER BIOMARKERS
卷 6, 期 5-6, 页码 281-290出版社
IOS PRESS
DOI: 10.3233/CBM-2009-0140
关键词
Antibody microarray; multianalyte; multiplex; high-throughput; biomarker; calibration
类别
资金
- NIH [CA117378, EB006177]
- NATIONAL CANCER INSTITUTE [U01CA117378] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB006177] Funding Source: NIH RePORTER
Enzyme-linked immunosorbent assay (ELISA) microarray technology promises to be a powerful tool for detecting and validating protein biomarkers, especially panels of biomarkers. ELISA microarrays are capable of high-throughput analysis of multiple proteins using small sample volumes. In this chapter we review the literature on the use of antibody microarrays for biomarker discovery and validation. We also described the methodologies we employ to obtain high-quality data through protocol optimization and data calibration.
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