期刊
FASEB JOURNAL
卷 21, 期 1, 页码 167-178出版社
WILEY
DOI: 10.1096/fj.06-6711com
关键词
eosinophils; neutrophils; lipid bodies; lipid droplets; endoplasmic reticulum; U937 cells
资金
- NHLBI NIH HHS [R01 HL070270, HL70270] Funding Source: Medline
- NIAID NIH HHS [R01 AI022571-17, AI20241, R37 AI020241, AI22571, R01 AI022571-21A2, R01 AI020241, AI33372, R01 AI022571, R01 AI022571-19, R01 AI033372, R01 AI022571-20, R01 AI022571-18] Funding Source: Medline
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL070270] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI033372, R01AI022571, R37AI020241, R01AI020241] Funding Source: NIH RePORTER
Lipid bodies (LBs), multifunctional organelles present in most eukaryotic cells, are sites of eicosanoid formation in leukocytes; but little is known about the composition of leukocyte LBs or the biogenesis and internal structures of LBs from mammalian cells. Proteomic analyses of LBs purified from human monocytic U937 cells detected, common to LBs in other cells, proteins involved in cholesterol and triglyceride metabolism, Rab GTPases, and many membrane and endoplasmic reticulum (ER)-associated proteins. Newly lipid body (LB)-associated proteins included MRP-14, potentially involved in arachidonate transport, and ribosomal subunit proteins and translation regulatory proteins. Ultrastructurally, in U937 cells as well as human neutrophils and eosinophils, ribosomes are attached to and distributed within LBs, and LBs contain extensive ER-like membranes. The presence of ribosomes, ER-like membranes and many membrane-associated and ER luminal proteins within LBs, supports a new model by which enveloped ER-membranes and domains form LBs and indicates that LBs may be sites of protein synthesis.
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