期刊
CANCER BIOLOGY & THERAPY
卷 8, 期 4, 页码 382-390出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/cbt.8.4.7453
关键词
proanthocyanidins; intestinal epithelial cell; Dark Agouti rat; 5-fluorouracil; anti-inflammation
类别
资金
- Cancer Council South Australia Research Fellowship
Mucositis is a common side-effect of high-dose chemotherapy regimens. Grape seed extract (GSE) represents a rich source of proanthocyanidins with the potential to decrease oxidative damage and inflammation within the gastrointestinal tract. We evaluated GSE for its capacity to decrease the severity of chemotherapy-induced mucositis in vitro and in vivo. In vitro: GSE was administered to IEC-6 intestinal epithelial cells prior to damage induced by 5-Fluorouracil (5-FU). Cell viability was determined by neutral red assay. In vivo: Female Dark Agouti rats (130-180 g) were gavaged with 1 ml GSE (400 mg/kg) daily (day 3-11) and received 5-FU (150 mg/kg) by intraperitoneal (i.p.) injection on day nine to induce mucositis. Rats were sacrificed at day 12 and intestinal tissues collected for myeloperoxidase and sucrase activity assays and histological analyses. Statistical analysis was performed by one-way ANOVA. GSE prevented the decrease in IEC-6 cell viability induced by 5-FU (p<0.01). Compared with 5-FU controls, GSE significantly reduced myeloperoxidase activity by 86% and 27% in the proximal jejunum (p<0.001) and distal ileum (p<0.05) respectively; decreased qualitative histological scores of damage (p<0.05) in the proximal jejunum; increased villus height in the proximal jejunum (17%; p<0.05) and distal ileum (50%; p<0.01), and attenuated the 5-FU-induced reduction of mucosal thickness by 16% in the jejunum (p<0.05) and 45% in the ileum (p<0.01). GSE partially protected IEC-6 cells from 5-FU-induced cytotoxicity and ameliorated intestinal damage induced by 5-FU in rats. GSE may represent a promising prophylactic adjunct to conventional chemotherapy for preventing intestinal mucositis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据