Stereochemistry of rHint1 hydrolase assisted cleavage of P-N bond in nucleoside 5 '-O-phosphoramidothioates

The Hint-1 hydrolase assisted cleavage of the P-N bond in adenosine-5'-O-[N-(tryptophanylamide)]phosphoramidothioate proceeds with retention of configuration at the phosphorus atom which is consistent with the formation of a covalent enzyme-substrate complex.