期刊
CANCER
卷 117, 期 17, 页码 4092-4099出版社
WILEY-BLACKWELL
DOI: 10.1002/cncr.26021
关键词
multinucleated cells; senescent-like cancer cells; cancer-initiating cells
类别
资金
- Center of Nuclear Receptor and Cell Signaling of the University of Houston
- Cancer Center Support Core [CA16672]
- National Cancer Institute of the National Institutes of Health [1U54-CA143837-01]
BACKGROUND: Large multinucleated cells (MNCs) commonly exist in tumorigenic cancer cell lines that are used widely in research. However, the contributions of MNCs to tumorigenesis are unknown. METHODS: In this study, MNCs were characterized in the murine fibrosarcoma cell line UV-2237 in vitro and in vivo at the single-cell level. RESULTS: The authors observed that MNCs originated from a rare subpopulation of mononuclear cells and were positive for a senescent marker, b-galactosidase. In addition, MNCs were responsible for the majority of clonogenic activity when cultured in hard agar; they were more resistant to chemotherapeutic agents than mononuclear cells; they could undergo asymmetric division (producing mononuclear cells) and self-renewal in vitro and in vivo; and, most important; a single MNC produced orthotopic, subcutaneous tumors (composed mainly of mononuclear cells) that gave rise to spontaneous lung metastases in nude mice. CONCLUSIONS: The current results indicated that the growth of MNCs may be arrested under stress and that MNCs are highly resistant to chemotherapy and can generate clonal, orthotopic, metastatic tumors. Cancer 2011;117:4092-9. (C) 2011 American Cancer Society.
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