期刊
CHEMOSPHERE
卷 66, 期 9, 页码 1618-1626出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2006.08.004
关键词
benzo[alpyrene; laccase; oxidation; metabolites; mediator; immobilization
资金
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [S06GM008047] Funding Source: NIH RePORTER
- NIGMS NIH HHS [S06GM08047] Funding Source: Medline
Laccase from Trametes versicolor was immobilized on nanoparticles and kaolinite by physical adsorption or chemical covalence in which the supporters were activated by cross-linked with glutaraldehyde. Thermal and pH stabilities of immobilized laccase on these different supporters were compared. The degradation efficiencies of these immobilized laccases on oxidation of benzo[a]pyrene (BaP) were also compared. The results showed that the immobilized laccases on nanoparticles were more stable in resisting pH and thermal changes. After 48 It oxidation, laccase immobilized on kaolinite using the covalent coupling method showed a higher efficiency of oxidation with the BaP residue of 23% in the presence of I mM HBT and with BaP residue of 37% in I mM ABTS as the mediator. The results also exhibited a significant inhibition by 1% surfactant Tween 80. According to the HPLC analysis, the oxidation products including 1,6-benzo[a]pyrene quinone, 3,6-benzo[a]pyrene quinone and 6,12-benzo[a]pyrene quinone were identified. (c) 2006 Elsevier Ltd. All rights reserved.
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