期刊
FOOD CHEMISTRY
卷 100, 期 4, 页码 1704-1712出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2005.10.016
关键词
procyanidin-rich grape seed extract; Caco-2 cells; in vitro digestion; proteins interactions
Although the bioactivity of flavonoids is related to their bioavailability, little is known about pre-absorption events in the gastrointestinal tract and their possible interactions with digestive constituents and intestinal cells. Using an in vitro digestion/Caco-2 cell culture model, we investigated the effect of digestive secretions on the stability of (+)-catechin (CAT), (-)-epicatechin (EC) and B2 and B3 dimers from a procyanidin-rich grape seed extract (GSE). The availability of phenolic compounds was not affected by salivary and gastric incubations but decreased during intestinal digestion in the absence of Caco-2 cells due to interactions with pancreatic proteins, unmasked by acetonitrile extraction. Then, in the presence of cells, about 43.9% of CAT, 85.3% of EC and all dimers disappeared at the end of 2 h of intestinal incubation. The stability of all compounds at intestinal pH was demonstrated as well as interactions with proteins, associated with a decrease of some cells enzyme activities, e.g., alkaline phosphatase (-79.8%), sucrase-isomaltase (-60.9%) and aminopeptidase N (-60.7%). Moreover, no compounds were detected in the basal compartment of transwells or in cell monolayers. (c) 2005 Elsevier Ltd. All rights reserved.
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