期刊
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
卷 42, 期 3, 页码 572-581出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.yjmcc.2006.11.018
关键词
spectrin; fodrin; connexin 43; gap junctions; cytoskeleton; JNK
资金
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL075106, R01HL108186, P01HL070709, R01HL070079, R01HL103205] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM008181] Funding Source: NIH RePORTER
- NHLBI NIH HHS [R01 HL075106, R01 HL103205, R01 HL108186, P01 HL070709-05, R01 HL070079-04, R01 HL075106-03, P01 HL070709, R01 HL070079] Funding Source: Medline
- NIGMS NIH HHS [T32 GM008181, T32 GM008181-20] Funding Source: Medline
Decreases in the expression of connexin 43 and the integrity of gap junctions in cardiac muscle, induced by the constitutive activation of the c-Jun N-terminal kinase (JNK) signaling pathway, have been linked to conduction defects and sudden cardiac failure in mice [Petrich BG, Gong X, Lerner DL, Wang X, Brown JH, Saffitz JE, Wang Y. c-Jun N-terminal kinase activation mediates downregulation of connexin 43 in cardiomyocytes. Circ Res. 91 (2002) 640-647; B.G. Petrich, B.C. Eloff, D.L. Lerner, A. Kovacs, J.E. Saffitz, D.S. Rosenbaum, Y. Wang, Targeted activation of c-Jun N-terminal kinase in vivo induces restrictive cardiomyopathy and conduction defects. J. Biol. Chem. 2004;279: 15330-15338]. We examined the membrane cytoskeletal protein, all-spectrin, which associates with connexin 43, to learn if changes in its association with connexin 43 are linked to the instability of gap junctions. Several forms of all-spectrin are expressed in the heart, including one, termed alpha II-SH3i, which contains a 20-amino-acid sequence next to the SH3 domain of repeat 10. In adult mouse heart, antibodies to all forms of alpha II-spectrin labeled the sarcolemma, transverse (t-) tubules and intercalated disks of cardiomyocytes. In contrast, antibodies specific for alpha II-SH3i labeled only gap junctions and transverse tubules. In transgenic hearts, in which the JNK pathway was constitutively activated, alpha II-SH3i was lost specifically from gap junctions but not from t-tubules while other isoforms of alpha II-spectrin were retained at intercalated disks. Immunoprecipitations confirmed the decreased association of alpha II-SH3i with connexin 43 in transgenic hearts compared to controls. Furthermore, activation of JNK in neonatal myocytes blocked the formation of gap junctions by exogenously expressed Cx43-GFP fusion protein. Similarly, overexpression of the SH3i fragment in the context of repeats 9-11 of alpha II-spectrin specifically caused the accumulation of Cx43-GFP in the perinuclear region and inhibited its accumulation at gap junctions. These results support a critical role for the alpha II-SH3i isoform of spectrin in intracellular targeting of Cx43 to gap junctions and implicates alpha II-SH3i as a potential target for stress signaling pathways that modulate intercellular communication. (c) 2006 Elsevier Inc. All rights reserved.
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