期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 387, 期 1, 页码 63-69出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-006-0403-8
关键词
microscopy; imaging; mast cells; CCD camera
We demonstrate the capability of differential interference contrast (DIC) microscopy as a simple and useful tool for studying cellular events without fluorescence labeling. By coupling an advanced DIC microscope to a computer-controlled motorized vertical stage and a high-speed, high-resolution CCD camera, real-time three-dimensional monitoring is possible in a high-throughput manner. The performance among three modes of microscopy, bright-field, dark-field and DIC, in terms of horizontal resolving power and vertical sectioning was investigated. As a model, exocytosis of rat peritoneal mast cells was recorded on the subsecond time scale. Three-dimensional tracking of granules during degranulation was achieved and granule-granule fusion before plasma membrane fusion was recorded.
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