期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 51, 期 1, 页码 252-256出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.00892-06
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资金
- FIC NIH HHS [T37 TW000048, 5T37TW000048-07] Funding Source: Medline
- NIAID NIH HHS [R15 AI047115, AI47115-02] Funding Source: Medline
- NIGMS NIH HHS [R25 GM56820-03, R25 GM056820] Funding Source: Medline
- NIMHD NIH HHS [T37 MD001368, 5T37MD001368-09] Funding Source: Medline
- FOGARTY INTERNATIONAL CENTER [T37TW000048] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R15AI047115] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R25GM056820] Funding Source: NIH RePORTER
- National Institute on Minority Health and Health Disparities [T37MD001368] Funding Source: NIH RePORTER
Aminoglycosides bind to the 16S rRNA at the tRNA acceptor site (A site) and disturb protein synthesis by inducing codon misreading. We investigated Escherichia coli cell elongation and division, as well as the dynamics of chromosome replication and segregation, in the presence of sublethal concentrations of amikacin (AMK). The fates of the chromosome ori and ter loci were monitored by visualization by using derivatives of LacI and TetR fused to fluorescent proteins in E. coli strains that carry operator arrays at the appropriate locations. The results showed that cultures containing sublethal concentrations of AMK contained abnormally elongated cells. The chromosomes in these cells were properly located, suggesting that the dynamics of replication and segregation were normal. FtsZ, an essential protein in the process of cell division, was studied by using an ectopic FtsZ-cyan fluorescent protein fusion. Consistent with a defect in cell division, we revealed that the Z ring failed to properly assemble in these elongated cells.
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