期刊
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
卷 92, 期 1, 页码 70-76出版社
ENDOCRINE SOC
DOI: 10.1210/jc.2006-1450
关键词
-
Context: Terminal differentiation of the human thyroid is characterized by the onset of follicle formation and thyroid hormone synthesis at 11 gestational weeks (GW). Objective: This study aimed to investigate the ontogeny of thyroglobulin (Tg), thyroid peroxidase (TPO), sodium/iodide symporter (NIS), pendrin (PDS), dual oxidase 2 (DUOX2), thyroid-stimulating hormone receptor (TSHR), and thyroid transcription factor 1 (TITF1), forkhead box E1 (FOXE1), and paired box gene 8 (PAX8) in the developing human thyroid. Design: Thyroid tissues from human embryos and fetuses (7-33 GW; n = 45) were analyzed by quantitative PCR to monitor mRNA expression for each gene and by immunohistochemistry to determine the cellular distribution of TITF1, TSHR, Tg, TPO, NIS, and the onset of T-4 production. A broken line regression model was fitted for each gene to compare the loglinear increase in expression before and after the onset of T-4 synthesis. Results: TITF1, FOXE1, PAX8, TSHR, and DUOX2 were stably expressed from 7 to 33 GW. Tg, TPO, and PDS expression was detectable as early as 7GW and was correlated with gestational age (all, P < 0.01), and the slope of the regression line was significantly different before and after the onset of T-4 synthesis at 11 GW (all, P < 0.01). NIS expression appeared last and showed the highest fit by the broken line regression model of all genes (correlation age P < 0.0001, broken line regression P < 0.0001). Immunohistochemical studies detected TITF1, TSHR, and Tg in unpolarized thyrocytes before follicle formation. T-4 and NIS labeling were only found in developing follicles from 11 GW on. Conclusion: These results imply a key role of NIS for the onset of human thyroid function.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据