期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 13, 期 14, 页码 3878-3885出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200601372
关键词
cellular uptake; drug delivery; iron; nanorods; polyelectrolytes
A low-temperature solution approach (90-95 degrees C) using FeCl3 and urea was carried out to synthesize beta-FeOOH nanorods in aqueous solution. The as-synthesized beta-FeOOH nanorods were further calcined at 300 degrees C to form porous nanorods with compositions including both beta-FeOOH and alpha-Fe2O3. The derived porous nanorods were engineered to assemble with four layers of polyelectrolytes (polyacrylic acid (PAA)/polyethylenimine(PEI)/PAA/ PEI) on their surfaces as polyelectrolyte multilayer nanocapsules. Fluorescein isothiocyanate (FITC) molecules were loaded into the polyelectrolyte multilayer nanocapsules in order to investigate drug release and intracellular delivery in Hela cells. The as-prepared nanocapsules showed ionic strength-dependent control of the permeability of the polyelectrolyte shells. The release behavior of the entrapped FITC from the FITC-loaded nanocapsules exhibited either controlled- or sustained-release trends, depending on the compactness of the polyelectrolyte shells on the nanorod surfaces. Cytotoxicity measurements demonstrate that the native nanorods and the polymercoated nanorods have excellent biocompatibility in all dosages between 0.1 ng mL(-1) and 100 mu gmL(-1). The time dependence of uptake of FITC-loaded nanocapsules by Hela cancer cells observed by laser confocal microscopy indicates that the nanocapsules can readily be taken up by cancer cells in 15 min, a relatively short period of time, while the slow release of the FITC from the initial perimembrane space into the cytoplasm was followed by release into the nucleus after 24 h.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据