期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 13, 期 17, 页码 5018-5023出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200601605
关键词
circular dichroism; DNA recognition; humna telometes; mass spectrometry; quadruplexes
Electrospray ionization mass spectrometry (ESI-MS) was utilized to investigate the binding affinity and stoichiometry of small molecules with human telomeric G-quadruplex DNA. The binding-affinity order obtained for the (AGGGTT)(4) quadruplex was: Te101 > ImImIm beta Dp >> PyPyPy gamma ImIm-Im beta Dp. The specific binding of Te101 and PyPyPy gamma ImImIm beta Dp in one system consisting of human telomeric G-quadruplex and duplex DNA was observed directly for the first time. This revealed that PyPyPy gamma ImImIm beta Dp has a binding specificity for the duplex DNA, whereas Te101 selectively recognizes the G-quadruplex DNA. Moreover, both ESI-MS and circular dichroism (CD) spectra indicated that Te101 favored the formation and stabilization of the antiparallel G-quadruplex, and a structural transition of the (AGGGTT)(4) sequence from a coexistence of parallel and antiparallel G-quadruplexes to a parallel G-quadruplex induced by annealing.
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