期刊
KIDNEY INTERNATIONAL
卷 71, 期 1, 页码 74-78出版社
ELSEVIER SCIENCE INC
DOI: 10.1038/sj.ki.5001988
关键词
plasma creatinine; determination methods; enzymatic; HPLC; colorimetric; Jaffe
The use of the colorimetric Jaffe method for the measurement of creatinine in mouse and rat plasma has been criticized as prior studies have shown a dramatic overestimation. We compared a colorimetric picric acid, an enzymatic, and a high-performance liquid chromatography (HPLC) method to assess their appropriateness for routine measurements of creatinine in plasma of healthy and diseased mice (n = 61) and rats (n = 56). For the colorimetric Jaffe method a pronounced overestimation is confirmed. Additionally the method showed interference with hemoglobin already in a very low, non-visible concentration range in rat plasma. The enzymatic measurement demonstrated a hemoglobin interference in mice, only when hemolysis was visible. The comparison between HPLC and the enzymatic measurement gave a good agreement between both methods in both species. Therefore the enzymatic method fulfills the requirements for a routine screening test for plasma creatinine in healthy as well as diseased mice and rats over a broad concentration range.
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