Bodipy-VAD-Fmk, a useful tool to study yeast peptide N-glycanase activity

In this paper the development of a fluorescent activity based probe, Bodipy-VAD- Fmk, for visualization of yeast peptide N-glycanase is described. The activity based probe is used to assess the efficacy of known and new chitobiose-based electrophilic traps to bind yeast peptide N-glycanase.