期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 73, 期 6, 页码 1275-1281出版社
SPRINGER
DOI: 10.1007/s00253-006-0619-8
关键词
N-p-nitrophenyl-3-ketovalidamine; 3-ketovalidoxylamine A C-N lyase; ethylenediaminetetraacetic acid; glucoside 3-dehydrogenase
3-Ketovalidoxylamine A C-N lyase is one of three key enzymes in the production of valienamine, which is a potent glucosidase inhibitor from validamycin A. N-p-Nitrophenyl-3-ketovalidamine, used as the substrate of 3-ketovalidoxylamine A C-N lyase, was prepared from N-p-nitrophenylvalidamine with free cells of Stenotrophomonas maltrophilia CCTCC M 204024. The yield and selectivity of N-p-nitrophenyl-3-ketovalidamine from cells were improved by treatment with 10 mM ethylenediaminetetraacetic acid. The optimal pH and temperature for N-p-nitrophenyl-3-ketovalidamine formation was pH 6.0 and 30 degrees C, respectively. N-p-Nitrophenyl-3-ketovalidamine was formed with a yield of 0.68 in the first batch.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据