期刊
ORGANIC & BIOMOLECULAR CHEMISTRY
卷 5, 期 15, 页码 2458-2463出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b706507h
关键词
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资金
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM076360] Funding Source: NIH RePORTER
- NIGMS NIH HHS [R01 GM 076360, R01 GM076360-02, R01 GM076360] Funding Source: Medline
Sialyl Tn (STn) and sialyl lactoside derivatives containing O-acetylated sialic acid residues have been chemoenzymatically synthesized using a one-pot three-enzyme system and conjugated to biotinylated human serum albumin (HSA) using an adipic acid para-nitrophenyl ester coupling reagent. This approach provides an efficient and general protocol for preparing carbohydrate-protein conjugates containing base-sensitive groups.
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