期刊
FEMS MICROBIOLOGY LETTERS
卷 271, 期 2, 页码 207-213出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1574-6968.2007.00718.x
关键词
carbofuran; biodegradation; Novosphingobium sp.; novel metabolite
类别
A gram-negative Novosphingobium sp. strain FND-3 capable of degrading carbofuran was isolated and characterized. The carbofuran-degrading ability of strain FND-3 was investigated under various culture conditions. Strain FND-3 showed a high average carbofuran-degrading rate of 28.6 mg L-1 h(-1) in mineral salts medium with 100 mg L-1 carbofuran. GC/MS analysis pointed out the presence of several unknown metabolites. One hydrolyzate was identified as 2-hydroxy-3-(3-methypropan-2-ol) phenol via hydrolysis of carbofuran phenol. The appearance of another metabolite with M+ of 180 m/z indicated that the hydroxylation of carbofuran occurred at the aromatic ring. One novel degrading product with M+ of 239 m/z was identified as 2-hydroxy-3-(3-methylpropan-2-ol) benzene-N-methylcarbamate via hydrolyzing at the ether bond of furanyl ring of carbofuran. Strain FND-3 was also able to degrade other N-methylcarbamate pesticides.
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