期刊
JOURNAL OF CELL SCIENCE
卷 120, 期 1, 页码 125-136出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.03317
关键词
keratinocyte; differentiation; SUMO; HaCaT; Ubc9
类别
资金
- NCI NIH HHS [R01 CA085747, CA089298, R01 CA089298] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R01CA089298, R01CA085747] Funding Source: NIH RePORTER
SUMO modification regulates the activity of numerous transcription factors that have a direct role in cell-cycle progression, apoptosis, cellular proliferation, and development, but its role in differentiation processes is less clear. Keratinocyte differentiation requires the coordinated activation of a series of transcription factors, and as several crucial keratinocyte transcription factors are known to be SUMO substrates, we investigated the role of sumoylation in keratinocyte differentiation. In a human keratinocyte cell line model (HaCaT cells), Ca2+-induced differentiation led to the transient and coordinated transcriptional activation of the genes encoding crucial sumoylation system components, including SAE1, SAE2, Ubc9, SENP1, Miz-1 (PIASx beta), SUMO2 and SUMO3. The increased gene expression resulted in higher levels of the respective proteins and changes in the pattern of sumoylated substrate proteins during the differentiation process. Similarly to the HaCaT results, stratified human foreskin keratinocytes showed an upregulation of Ubc9 in the suprabasal layers. Abrogation of sumoylation by Gam1 expression severely disrupted normal HaCaT differentiation, consistent with an important role for sumoylation in the proper progression of this biological process.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据